Classification: | Biochemical Reagents |
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Specific Usage: | For Biological Purpose, For Tissue Medium Purpose |
Content: | Standard |
Usage: | Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents |
Source: | Extract Natural Things |
Habit Appellation: | Chemical Medicine |
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HF502C is a serum-free and protein-free medium suitable for insect cell culture, with a dry powder containing Pluronic F-68, glutamine and sodium bicarbonate. Optimized to support the high-density suspension growth of the BTI-TN-5BM (Tn5, High5) cell line and the recombinant gene expression of the baculovirus expression system (BEVs). Furthermore, HF502C supports the production of recombinant proteins by High5 cells. The highest cell density of H5 cells in HF502C can achieve 9.0×106 cells/ml, which can be passaged more than 20 times without reduced activity. HF502C is a complete medium that does not require additional protein or other insect hormones before use.
Supports the Baculovirus Expression Vector System (BEVS) for recombinant gene expression |
Stable protein expression |
Easy purification |
No need for protein or other insect hormone supplementation |
Transported at room temperature, stored at 2-8°C, valid for 3-6 months.
In order to maintain the best results, please do not freeze-thaw.1.Add 90% final volume of ultrapure water to the suitable container, add the suitable culture medium to stir and dissolve, and add the ultrapure water to the final volume.
2.Adjust pH to 6.0-6.4 with 10N sodium hydroxide or concentrated hydrochloric acid solution as required
3.The bacteria were removed by 0.2 um micron filter membrane. and the solution should be kept away fromlight at 2-8°C.
4.1ml of component B (or sterile added after filtration) to each liter of the medium before filtration
One of the leading biopharmaceutical one-stop solution providers in China
Suzhou Womei Biology Co., Ltd (Womei Biology) is one of the leading one-stop solution providers for biopharmaceuticals in China, with products including personalized media, bioreactors, immunoadjuvants, biochemical reagents and other bioprocesses upstream and downstream core suppliers, as well as providing formulations, process optimization, process validation, technical support and ancillary services for biopharmaceutical industry. We also provide formulation production, process optimization, process validation, technical support and supporting services for the biopharmaceutical industry. Now it is one of the largest high-tech integrated suppliers in the field of animal pet disease manufacturing, and also ranks the top in the whole biopharmaceutical upstream field.----------------------------------------------------------------------------------------------------------------------------------
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